Mark Buchheim, PhD

Mark Buchheim, PhD
Professor of Biological Science
College of Engineering & Natural Sciences
Biological Science
918-631-3833 Website Oliphant Hall Room 312

Education

PhD – University of Illinois, Urbana-Champaign MS – Iowa State University BA – Wartburg College

Bio

Mark Buchheim's primary research interests are focused on the phylogenetic reconstruction and molecular evolution of the green algae with particular emphasis on the genus Chlamydomonas and its allies (including Carteria, Chloromonas, and Chlorococcum). In addition, he remains interested in the enigmatic group of green algae known as the Sphaeropleaceae. His lab has been a leader in the use of ribosomal (18S, 28S and ITS) and chloroplast-encoded rbcL and atpB genes to infer phylogenetic relationships in the green algae. Buchheim served as PI and member of the Executive Committee of Deep Green, a USDA/NSF/DOE-funded project involving the coordination of research effort in green plant phylogeny (i.e., the study of phylogenetic relationships among the major green algal and embryophyte lineages). He also collaborated on a multi-investigator project to study microbial diversity at the Great Salt Plains of Oklahoma. Current research interests include diversity of the blood alga (Haematococcus) rRNA secondary structure analysis, sequence-structure data in phylogenetic reconstruction, rRNA intragenomic variation and the relationship between concerted evolution and sexual reproduction in green algae. In addition, graduate research in the lab has focused on Aspergillus diversity, molecular characterization of Juniperus pollen and the design of microbial consortia for wastewater remediation.

Research Interests

Eukaryotic Microbial Diversity
Phylogenetics
rRNA Evolution
Green Algal Systematics
Aquatic Biology

Teaching Interests

Introductory Biology
Evolution
Algal Biology
Plant Diversity

Publications

Journal Articles

  • 1.
    Marasini D, Karki A, Buchheim M, Fakhr M. Phylogenetic Relatedness Among Plasmids Harbored by Campylobacter jejuni and Campylobacter coli Isolated From Retail Meats. Frontiers in microbiology; 2018;9:2167.
  • Wolf, M. & Buchheim, M. A. 2018. The internal transcribed spacer 2 of Jenufa (Chlorophyta, Chlorophyceae) is extraordinarily long: A hypothesis. Gene 678: 100-104.

  • Henley, W. J., Cobbs, M., Novoveská, L., & Buchheim, M. A. 2018. Phylogenetic analysis of Dunaliella (Chlorophyta) emphasizing new benthic and supralittoral isolates from Great Salt Lake. Journal of Phycology doi: 10.1111/jpy.12747.

  • Polle, J. E., Barry, K., Cushman, J., Schmutz, J., Tran, D., Hathwaik, L. T., Yim, W. C., Jenkins, J., McKie-Krisberg, Z., Prochnik, S., Lindquist, E., Dockter, R. B., Adam, C., Molina, H., Bunkenborg, J., Jin, E., Buchheim, M. A. & Magnuson, J. 2017. Draft genome sequence of the halophilic and beta-carotene-accumulating green alga Dunaliella salina strain CCAP19/18. Genome Announcements DOI:10.1128/genomeA.01105-17.

  • Alanagreh, L., Pegg, C., Harikumar, A. & Buchheim. M. A. 2017. Assessing intragenomic variation of the internal transcribed spacer two: Adapting the Illumina metagenomics protocol. PLoS ONE 12(7): e0181491

  • Mohanty, R. P., Buchheim, M. A., Anderson, J. & Levetin, E. 2017. Molecular analysis confirms the long distance transport of Juniperus ashei pollen. PLoS ONE 12 (3): e0173465.

  • Buchheim M, Muller T, Wolf M. 18S rDNA sequence-structure phylogeny of the Chlorophyceae with special emphasis on the Sphaeropleales. Plant Gene. Plant Gene; 2017;10:45–50.

  • Mohanty, R. P., Buchheim, M. A. & Levetin, E. 2017. Molecular approaches for the analysis of airborne pollen: A case study of Juniperus pollen. Annals of Allergy, Asthma and Immunology 118 (2): 204-211

  • Marin Rodrigues, M. V., Müller, T., Buchheim, M. A., Canbäck, B. & Wolf, M. 2017. THESES db: The algae 18S rDNA sequence-structure database for inferring phylogenies. Phycologia 56 (2):186-192.

  • Mohanty, Rashi et al. “Molecular and Ultrastructural Detection of Plastids in Juniperus (Cupressaceae) Pollen.” Phytologia 98.4 (2016): 298–310. Print.

  • *Pegg, C., Wolf, M., †Alanagreh, L., Portman, R. & Buchheim, M. A. 2015. Morphological diversity masks phylogenetic similarity of Ettlia and Haematococcus (Chlorophyceae). Phycologia 54 (4):385-397.

  • Assunção, P., Jaén-Molina, R., Caujapé-Castells, J., Wolf, M., Buchheim, M. A., de la Jara, A., Freijanes, K., Carmona, L. & Mendoza, H. 2013. Phylogenetic analysis of ITS2 sequences suggests the taxonomic re-structuring of Dunaliella viridis (Chlorophyceae, Dunaliellales). Phycological Research 61(2):81-88.

  • Buchheim, M. A., *Sutherland, D. M., Buchheim, J. A. & Wolf, M. 2013. The blood alga: Phylogeny of Haematococcus (Chlorophyceae) inferred from ribosomal RNA gene sequence data. European Journal of Phycology 48(3):318-329.

  • Buchheim, M. A., *Sutherland, D. M., Schleicher, T., Förster, F. & Wolf, M. 2012. Phylogeny of the Oedogoniales, Chaetophorales and Chaetopeltidales (Chlorophyceae): Inferences from sequence-structure analysis of ITS2. Annals of Botany 109:109-116.

  • Buchheim, M. A., Keller, A., Koetschan, C., Förster, F., Merget, B & Wolf, M. 2011. Internal transcribed spacer 2 (nu ITS2 rRNA) sequence-structure phylogenetics: Towards an automated reconstruction of the green algal tree of life. PLoS One 6: e16931. doi:10.1371/journal.pone.0016931

  • Buchheim, M. A., *Sutherland, D. M., Schleicher, T., Förster, F. & Wolf, M. 2012. Phylogeny of the Oedogoniales, Chaetophorales and Chaetopeltidales (Chlorophyceae): Inferences from sequence-structure analysis of ITS2. Annals of Botany 109:109-116.

  • Zechman, F. W., Verbruggen, H., Buchheim, M. A., Fawley, M. W., Ashworth, M., Leliaert, F., Spalding, H., Pueschel, C. M., Buchheim, J. A., †Verghese, B. & Hanisak, M. D. 2010. An unrecognized lineage of green plants persists in deep marine waters. Journal of Phycology 46 (6):1288-1295. doi: 10.1111/j.1529-8817.2010.00900.x

  • Buchheim, M. A., Kirkwood, A., †Verghese, B, Buchheim, J. and Henley W. 2010. Hypersaline soil supports a diversified community of Dunaliella (Chlorophyceae). Journal of Phycology 46 (5):1038-47.

  • Kirkwood, A. E., Buchheim, J. A., Buchheim, M. A. and Henley, W. J. 2008. Cyanobacteria diversity and halotolerance in a variable hypersaline environment. Microbial Ecology. Online: doi 10.1007/s00248-007-9291-5

  • Padhi, A., Buchheim, M. A., †Verghese, B. 2008. Dynamic evolutionary pattern of alpha-2 macroglobulin in a model organism, the Zebrafish (Danio rerio). Molecular Immunology 45: 3312-3318.

  • Buchheim, M. A., Buchheim, J. A., *Carlson, T., Braband, A., Hepperle, D., Krienitz, L., Wolf, M., & Hegewald, E. 2005. Phylogeny of the Hydrodictyaceae (Chlorophyceae): Inferences from rDNA data. Journal of Phycology 41:1039-1054.

  • Wilson, C., Caton, T.M., Buchheim, J.A., Buchheim, M.A., Schneegurt, M.A. & Miller, R.V. 2004. DNA-repair potential in Halomonas spp. from the Salt Plains Microbial Observatory of Oklahoma. Microbial Ecology 48:541-549.

  • Caton, T. M. Witte, L. R, Ngyuen, H. D., Buchheim, J. A., Buchheim, M. A., & Schneegurt, M. A. 2004. Halotolerant Aerobic Heterotrophic Bacteria from the Great Salt Plains of Oklahoma. Microbial Ecology 48:449-62.

  • Fawley, M. W., Fawley, K. P. & Buchheim, M. A. 2004. Molecular diversity among communities of freshwater microchlorophytes. Microbial Ecology 48:489-99.

  • Henley, W. J., Hironaka, .J. L., L. Guillou, L., Buchheim, M. A. Buchheim, J. A.,Fawley, M. W. & Fawley, K. P. 2004. Phylogenetic analysis of the “Nannochloris-like” algae and diagnoses of Picochlorum oklahomensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta). Phycologia 43(6): 641-652.

  • Wolf, M., Buchheim, M. A., Hegewald, E., Krienitz, L. & Hepperle, D. 2002. Phylogenetic position of the Sphaeropleaceae (Chlorophyta). Plant Systematics and Evolution 230:161-171.

  • Buchheim, M. A., Buchheim, J. A., *Carlson, T & Kugrens, P. 2002. Phylogeny of Lobocharacium (Chlorophyceae) and allies: A study of 18S and 26S rDNA data. Journal of Phycology 38:376-383.

  • Hill, Peggy S. M., Cara Hoffart and Mark Buchheim. 2002. Tracing phylogenetic relationships in the family Gryllotalpidae. Journal of Orthoptera Research, 11:169-174.

  • Buchheim, M. A., *†Michalopulos, E. A. & Buchheim, J. A. 2001. Phylogeny of the Chlorophyceae with special reference to the Sphaeropleales: A study of 18S and 26S rDNA data. Journal of Phycology 37:819-835.

  • Buchheim, M.A., Buchheim, J. A. & Chapman, R. L. 1997. Phylogeny of Chloromonas: A study of 18S ribosomal RNA gene sequences. Journal of Phycology 33:286-293.

  • Buchheim, M.A., Buchheim, J. A. & Chapman, R. L. 1997. Phylogeny of the VLE-14 Chlamydomonas (Chlorophyceae) group: A study of 18S rRNA gene sequences. Journal of Phycology 33:1024-1030.

  • Buchheim, M. A., Lemieux, C., Otis, C., Gutell, R.R., Chapman, R. L. and Turmel, M. 1996. Phylogeny of the Chlamydomonadales (Chlorophyceae): A comparison of ribosomal RNA gene sequences from the nucleus and the chloroplast. Molecular Phylogenetics and Evolution 5:391-402.

  • Fawley, M. & Buchheim, M.A. 1995. Loroxanthin, a phylogenetically useful character in Chlamydomonas and other chlorophycean flagellates. Journal of Phycology 31:664-667.

  • Buchheim, M. A., *McAuley, M. A., Zimmer, E. A., Theriot, E. C. & Chapman, R. L. 1994. Multiple origins of colonial green flagellates from unicells: Evidence from molecular and organismal characters. Molecular Phylogenetics and Evolution 3:322-343.

  • Mishler, B. D., Lewis, L. A., Buchheim, M. A., Renzaglia, K. S., Delwiche, C. F., Zechman, F. W., Kantz, T. S., and Chapman, R. L. 1994. Phylogenetic relationships of the “green algae” and bryophytes.” Annals of the Missouri Botanical Garden 81: 451-483.

  • Chapman, R. L. & Buchheim, M. A. 1992. Green algae and the evolution of land plants: Inferences from nuclear-encoded rRNA gene sequences. BioSystems 28:127-137.

  • Buchheim, M. A. & Chapman, R. L. 1992. Phylogeny of Carteria (Chlorophyceae) inferred from organismal and molecular data. Journal of Phycology 28:362-374.

  • Waters, D. A., Buchheim, M. A., Dewey, R. A. & Chapman, R. L. 1992. Preliminary inferences of the phylogeny of bryophytes from nuclear-encoded ribosomal RNA sequences. American Journal of Botany 79:459-466.

  • Buchheim, M. A. & Chapman, R. L. 1991. Phylogeny of the colonial green flagellates: A study of 18S and 26S rRNA sequence data. BioSystems, 25:85-100.

  • Buchheim, M. A., Turmel, M., Zimmer, E. A. & Chapman, R. L. 1990. Phylogeny of Chlamydomonas (Chlorophyta) based on cladistic analysis of nuclear 18S rRNA sequence data. Journal of Phycology, 26:689-699.

Book Chapters

  • Chapman, R. L., Buchheim, M. A., Delwiche, C. F., Friedl, T., Huss, V. A. R., Karol, K. G., Lewis, L. A., Manhart, J., McCourt, R. M., Olsen, J. L., & Waters, D. A. 1998. Molecular systematics of the green algae. In: Soltis, D & Soltis, P. [Eds.] Molecular Systematics of Plants II. Chapman-Hall, pp. 508-540.

Other

  • Hill, P. S. M., Hoffart, C. and Buchheim, M. 2002. Tracing relationships in the Gryllotalpidae. Integrative and Comparative Biology, 42:1244.

  • Buchheim, M. A. 2000. Green Algae. In: Encyclopedia of Life Sciences (online at www.els.net), Macmillan Reference Ltd.

Courses Taught

  • Research Experience
  • The Fungi and Algae
  • Independent Research
  • Evolution

Professional Affiliations

  • Botanical Society of America
  • National Association of Advisers for the Health Professions

Awards & Honors

  • Luigi Provasoli Award
  • Wartburg College Alumni Citation
  • Zelimir Schmidt Award for Outstanding Research