Ty Johannes, PhD

Tyler Johannes
Ty Johannes, PhD
Chairperson, Russell School of Chemical Engineering
Wayne Banes Rumley Associate Professor of Chemical Engineering
College of Engineering & Natural Sciences
Chemical Engineering
918-631-2947 Keplinger Hall Room 3375


PhD – University of Illinois MS – University of Illinois BS – Oklahoma State University

Research Interests

Dr. Johannes received his B.S. in Chemical Engineering from Oklahoma State University and his M.S. and Ph.D. in Chemical Engineering from the University of Illinois. He teaches a wide range of courses including traditional chemical engineering course, biomedical engineering and the science of cooking. His research focuses on directed evolution, synthetic biology, and algae biofuels. Dr. Johannes is a licensed professional engineer in Oklahoma.

Teaching Interests

Chemical Reactor Design
Process Component Design
Biochemical Engineering
Fluid Mechanics
Heat Transfer
Science of Cooking


Journal Articles

  • Key, Katherine et al. “Assessing BTEX Biodegradation Potential at a Refinery Using Molecular Biological Tools.” Groundwater Monitoring & Remediation 34.1 (2014): 35–48. Print.

  • Effects of Algae Extract on the Growth and Metabolism of E. Coli and S. Cerevisiae. Vol. 17, Electronic Journal of Biotechnology, 2014, pp. 14-18.
  • Noor-Mohammadi, Samaneh, Azadeh Pourmir, and Tyler Johannes. “Method for Assembling and Expressing Multiple Genes in the Nucleus of Microalgae.” Biotechnology letters 36.3 (2014): 561–566. Print.

  • Production and Characterization of Algae Extract from Chlamydomonas Reinhardtii. Vol. 17, Electronic Journal of Biotechnology, 2014, pp. 3–3.
  • Key, Katherine et al. “An In Situ Bioreactor for the Treatment of Petroleum Hydrocarbons in Groundwater.” Remediation Journal 23.3 (2013): 55–84. Print.

  • Pourmir, Azadeh, Samaneh Noor-Mohammadi, and Tyler Johannes. “Production of Xylitol by Recombinant Microalgae.” Journal of biotechnology 165.3 (2013): 178–183. Print.

  • Zou, Yaozhong et al. “Crystal Structures of Phosphite Dehydrogenase Provide Insights into Nicotinamide Cofactor Regeneration.” Biochemistry 51.21 (2012): 4263–4270. Print.

  • ZouY., ZhangH., BrunzelleJ., JohannesT., WoodyerR., HungJ., NairN., ZhaoH., and NairS. Crystal Structures of Phosphite Dehydrogenase Provide Insights into Nicotinamide Cofactor Regeneration. Vol. 51, Biochemistry, 2012, pp. 4263–4270.
  • Pourmir, Azadeh, and Tyler Johannes. “Directed Evolution: Selection of the Host Organism.” Computational and structural biotechnology journal 2.3 (2012): 1–7. Print.

  • HungJ., FogleE., ChristmanH., JohannesT., ZhaoH., and MetcalfW. Investigation of the Role of Arg301 Identified in the X-Ray Structure of Phosphite Dehydrogenase. Vol. 51, Biochemistry, 2012, pp. 4254–4262.
  • Noor-MohammadiS., PourmirA., and JohannesT. Method to Assemble and Integrate Biochemical Pathways into the Chloroplast Genome of Chlamydomonas Reinhardtii. Vol. 109, Biotechnology and bioengineering, 2012, pp. 2896–2903.
  • JohannesT., DeSienoM., GriffinB., ThomasP., KelleherN., MetcalfW., and ZhaoH. Deciphering the Late Biosynthetic Steps of Antimalarial Compound FR-900098. Vol. 17, Chemistry & biology, 2010, pp. 57–64.
  • NairN., ShaoZ., ZhaoH., SullivanR., McLachlanM., JohannesT., and ZhaoH. Biobutanol from Yeast: A Synergistic Genome and Protein Engineering Approach. The 2008 AIChE annual meeting, Philadelphia, PA, 2008.
  • EliotA., GriffinB., ThomasP., JohannesT., KelleherN., ZhaoH., and MetcalfW. Biosynthesis of the Potent Antimalarial Compound FR900098. Vol. 15, Chemistry & biology, 2008, p. 765.
  • EliotA., GriffinB., ThomasP., JohannesT., KelleherN., ZhaoH., and MetcalfW. Cloning, Expression, and Biochemical Characterization of Streptomyces Rubellomurinus Genes Required for Biosynthesis of Antimalarial Compound FR900098. Vol. 15, Chemistry & biology, 2008, pp. 765–770.
  • DeSienoM., JohannesT., and ZhaoH. Deciphering the Late Steps In FR900098 Biosynthesis. The 2008 Annual Meeting, 2008.
  • McLachlanM., JohannesT., and ZhaoH. Further Improvement of Phosphite Dehydrogenase Thermostability by Saturation Mutagenesis. Vol. 99, Biotechnology and bioengineering, 2008, pp. 268–274.
  • ChoiY., JohannesT., SimurdiakM., LuH., and ZhaoH. Heterologous Production of Spectinabilin and Analysis of the Biosynthesis Gene Cluster. 한국생물공학회 학술대회, 2008, pp. 43–43.
  • JohannesT., WoodyerR., and ZhaoH. High-Throughput Screening Methods for Oxidoreductases. Protein Science Encyclopedia, 2008.
  • JohannesT., WoodyerR., and ZhaoH. Efficient Regeneration of NADPH Using an Engineered Phosphite Dehydrogenase. Vol. 96, Biotechnology and bioengineering, 2007, pp. 18–26.
  • JohannesT., and ZhaoH. Directed Evolution of Enzymes and Biosynthetic Pathways. Vol. 9, Current opinion in microbiology, 2006, pp. 261–267.
  • JohannesT., WoodyerR., and ZhaoH. High-Throughput Screening Methods Developed for Oxidoreductases. Enzyme Assays, 2006, p. 77.
  • WoodyerR., JohannesT., and ZhaoH. Regeneration of Cofactors for Enzyme Biocatalysis. Enzyme technology, 2006, pp. 83–101.
  • JohannesT., WoodyerR., and ZhaoH. Directed Evolution of a Thermostable Phosphite Dehydrogenase for NAD (P) H Regeneration. Vol. 71, Applied and environmental microbiology, 2005, pp. 5728–5734.

Conference Proceedings

  • HawrylakP., NicholsW., LiX., JohannesT., DeCookR., MongoldK., CornellC., FlintL., and SinghA. “HydroSense: A Self-Powered Wireless Device for Monitoring Water Usage in Hotel Showers”. Global Humanitarian Technology Conference (GHTC), 2015 IEEE, 2015, pp. 314-20.

Book Chapters

  • JohannesT., SimurdiakM., and ZhaoH. “Biocatalysis”. Encyclopedia of Chemical Processing, 2006, pp. 101–110.

Technical Reports

  • Development of Green Fuels From Algae-The University of Tulsa. The University of Tulsa, Tulsa, OK, 2012.


  • JohannesT. Directed Evolution of Phosphite Dehydrogenase and Engineered Biosynthesis of FR-900098. UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN, 2008.
  • ZhaoH., MetcalfW., JohnannesT., and WoodyerR. Oxidoreductase Comprising Enhanced Thermostability for Use As Nicotinamide Adenine Dinucleotide Phosphate Regenerative Tool. 2008.
  • ZhaoH., McLachlanM., and JohannesT. Engineered Phosphite Dehydrogenase Mutants. 2008.

Courses Taught

  • Thermodynamics
  • Research and Dissertation
  • Independent Study
  • Introduction to Biomedical Engineering
  • Chemical Engineering Lab II
  • Introductory Fluid Mechanics
  • Research and Thesis
  • Master's Project
  • Introduction to Chemical Engineering
  • Process Component Design
  • Biomedical Engineering Seminar

Professional Affiliations

  • AIChE

Awards & Honors

  • 2018 Outstanding Teacher
  • Kermit E. Brown Award for Teaching Excellence
  • Tau Beta Pi Teaching Excellence Award
  • Outstanding Teacher Award