Dr. Johannes received his B.S. in Chemical Engineering from Oklahoma State University and his M.S. and Ph.D. in Chemical Engineering from the University of Illinois. He teaches a wide range of courses including traditional chemical engineering course, biomedical engineering and the science of cooking. His research focuses on directed evolution, synthetic biology, and algae biofuels. Dr. Johannes is a licensed professional engineer in Oklahoma.

Chemical Reactor Design
Process Component Design
Biochemical Engineering
Fluid Mechanics
Thermodynamics
Heat Transfer
Science of Cooking

Journal Articles

• Key, Katherine et al. “Assessing BTEX Biodegradation Potential at a Refinery Using Molecular Biological Tools.” Groundwater Monitoring & Remediation 34.1 (2014): 35–48. Print.

• Effects of Algae Extract on the Growth and Metabolism of E. Coli and S. Cerevisiae. Vol. 17, Electronic Journal of Biotechnology, 2014, pp. 14-18.

• Noor-Mohammadi, Samaneh, Azadeh Pourmir, and Tyler Johannes. “Method for Assembling and Expressing Multiple Genes in the Nucleus of Microalgae.” Biotechnology letters 36.3 (2014): 561–566. Print.

• Production and Characterization of Algae Extract from Chlamydomonas Reinhardtii. Vol. 17, Electronic Journal of Biotechnology, 2014, pp. 3–3.

• Key, Katherine et al. “An In Situ Bioreactor for the Treatment of Petroleum Hydrocarbons in Groundwater.” Remediation Journal 23.3 (2013): 55–84. Print.

• Pourmir, Azadeh, Samaneh Noor-Mohammadi, and Tyler Johannes. “Production of Xylitol by Recombinant Microalgae.” Journal of biotechnology 165.3 (2013): 178–183. Print.

• Zou, Yaozhong et al. “Crystal Structures of Phosphite Dehydrogenase Provide Insights into Nicotinamide Cofactor Regeneration.” Biochemistry 51.21 (2012): 4263–4270. Print.

• ZouY., ZhangH., BrunzelleJ., JohannesT., WoodyerR., HungJ., NairN., ZhaoH., and NairS. Crystal Structures of Phosphite Dehydrogenase Provide Insights into Nicotinamide Cofactor Regeneration. Vol. 51, Biochemistry, 2012, pp. 4263–4270.

• Pourmir, Azadeh, and Tyler Johannes. “Directed Evolution: Selection of the Host Organism.” Computational and structural biotechnology journal 2.3 (2012): 1–7. Print.

• HungJ., FogleE., ChristmanH., JohannesT., ZhaoH., and MetcalfW. Investigation of the Role of Arg301 Identified in the X-Ray Structure of Phosphite Dehydrogenase. Vol. 51, Biochemistry, 2012, pp. 4254–4262.
• Noor-MohammadiS., PourmirA., and JohannesT. Method to Assemble and Integrate Biochemical Pathways into the Chloroplast Genome of Chlamydomonas Reinhardtii. Vol. 109, Biotechnology and bioengineering, 2012, pp. 2896–2903.
• JohannesT., DeSienoM., GriffinB., ThomasP., KelleherN., MetcalfW., and ZhaoH. Deciphering the Late Biosynthetic Steps of Antimalarial Compound FR-900098. Vol. 17, Chemistry & biology, 2010, pp. 57–64.
• NairN., ShaoZ., ZhaoH., SullivanR., McLachlanM., JohannesT., and ZhaoH. Biobutanol from Yeast: A Synergistic Genome and Protein Engineering Approach. The 2008 AIChE annual meeting, Philadelphia, PA, 2008.
• EliotA., GriffinB., ThomasP., JohannesT., KelleherN., ZhaoH., and MetcalfW. Biosynthesis of the Potent Antimalarial Compound FR900098. Vol. 15, Chemistry & biology, 2008, p. 765.
• EliotA., GriffinB., ThomasP., JohannesT., KelleherN., ZhaoH., and MetcalfW. Cloning, Expression, and Biochemical Characterization of Streptomyces Rubellomurinus Genes Required for Biosynthesis of Antimalarial Compound FR900098. Vol. 15, Chemistry & biology, 2008, pp. 765–770.
• DeSienoM., JohannesT., and ZhaoH. Deciphering the Late Steps In FR900098 Biosynthesis. The 2008 Annual Meeting, 2008.
• McLachlanM., JohannesT., and ZhaoH. Further Improvement of Phosphite Dehydrogenase Thermostability by Saturation Mutagenesis. Vol. 99, Biotechnology and bioengineering, 2008, pp. 268–274.
• ChoiY., JohannesT., SimurdiakM., LuH., and ZhaoH. Heterologous Production of Spectinabilin and Analysis of the Biosynthesis Gene Cluster. 한국생물공학회 학술대회, 2008, pp. 43–43.
• JohannesT., WoodyerR., and ZhaoH. High-Throughput Screening Methods for Oxidoreductases. Protein Science Encyclopedia, 2008.
• JohannesT., WoodyerR., and ZhaoH. Efficient Regeneration of NADPH Using an Engineered Phosphite Dehydrogenase. Vol. 96, Biotechnology and bioengineering, 2007, pp. 18–26.
• JohannesT., and ZhaoH. Directed Evolution of Enzymes and Biosynthetic Pathways. Vol. 9, Current opinion in microbiology, 2006, pp. 261–267.
• JohannesT., WoodyerR., and ZhaoH. High-Throughput Screening Methods Developed for Oxidoreductases. Enzyme Assays, 2006, p. 77.
• WoodyerR., JohannesT., and ZhaoH. Regeneration of Cofactors for Enzyme Biocatalysis. Enzyme technology, 2006, pp. 83–101.
• JohannesT., WoodyerR., and ZhaoH. Directed Evolution of a Thermostable Phosphite Dehydrogenase for NAD (P) H Regeneration. Vol. 71, Applied and environmental microbiology, 2005, pp. 5728–5734.

Conference Proceedings

• HawrylakP., NicholsW., LiX., JohannesT., DeCookR., MongoldK., CornellC., FlintL., and SinghA. “HydroSense: A Self-Powered Wireless Device for Monitoring Water Usage in Hotel Showers”. Global Humanitarian Technology Conference (GHTC), 2015 IEEE, 2015, pp. 314-20.

Book Chapters

• JohannesT., SimurdiakM., and ZhaoH. “Biocatalysis”. Encyclopedia of Chemical Processing, 2006, pp. 101–110.

Technical Reports

• Development of Green Fuels From Algae-The University of Tulsa. The University of Tulsa, Tulsa, OK, 2012.

Other

• JohannesT. Directed Evolution of Phosphite Dehydrogenase and Engineered Biosynthesis of FR-900098. UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN, 2008.
• ZhaoH., MetcalfW., JohnannesT., and WoodyerR. Oxidoreductase Comprising Enhanced Thermostability for Use As Nicotinamide Adenine Dinucleotide Phosphate Regenerative Tool. 2008.
• ZhaoH., McLachlanM., and JohannesT. Engineered Phosphite Dehydrogenase Mutants. 2008.
• ZHAOH., WOODYERR., METCALFW., VAN DER DONKW., and JohannesT. ENGINEERED PHOSPHITE DEHYDROGENASE MUTANTS FOR NICOTINAMIDE COFACTOR REGENERATION. 2006.

• Research and Dissertation
• Independent Study
• Introduction to Biomedical Engineering
• Chemical Engineering Lab II
• Introductory Fluid Mechanics
• Research and Thesis
• Master's Project
• Introduction to Chemical Engineering
• Process Component Design
• Biomedical Engineering Seminar

• AIChE

• 2018 Outstanding Teacher
• Kermit E. Brown Award for Teaching Excellence
• Tau Beta Pi Teaching Excellence Award
• Outstanding Teacher Award