Ty Johannes PhD

Wellspring Associate Professor of Chemical Engineering College of Engineering & Natural Sciences
Russell School of Chemical Engineering
Keplinger Hall 3375 918-631-2947

Ph.D., University of Illinois
M.S., University of Illinois
B.S., Oklahoma State University

Directed Evolution
Synthetic Biology
Algae Biofuels

Chemical Reactor Design
Biochemical Engineering
Fluid Mechanics
Heat Transfer

The following may be selected publications rather than a comprehensive list.

Journal Articles

Key, Katherine et al. “Assessing BTEX Biodegradation Potential at a Refinery Using Molecular Biological Tools.” Groundwater Monitoring & Remediation 34.1 (2014): 35–48. Print.

Noor-Mohammadi, Samaneh, Azadeh Pourmir, and Tyler Johannes. “Method for Assembling and Expressing Multiple Genes in the Nucleus of Microalgae.” Biotechnology letters 36.3 (2014): 561–566. Print.

Kightlinger, Weston et al. “Production and Characterization of Algae Extract from Chlamydomonas Reinhardtii.” Electronic Journal of Biotechnology 17.1 (2014): 3–3. Print.

Chen, Kai et al. “Effects of Algae Extract on the Growth and Metabolism of E. Coli and S. Cerevisiae.” Electronic Journal of Biotechnology 17 (2014): 14–18. Print.

Key, Katherine et al. “An In Situ Bioreactor for the Treatment of Petroleum Hydrocarbons in Groundwater.” Remediation Journal 23.3 (2013): 55–84. Print.

Pourmir, Azadeh, Samaneh Noor-Mohammadi, and Tyler Johannes. “Production of Xylitol by Recombinant Microalgae.” Journal of biotechnology 165.3 (2013): 178–183. Print.

Noor-Mohammadi, Samaneh, Azadeh Pourmir, and Tyler Johannes. “Method to Assemble and Integrate Biochemical Pathways into the Chloroplast Genome of Chlamydomonas Reinhardtii.” Biotechnology and bioengineering 109.11 (2012): 2896–2903. Print.

Zou, Yaozhong et al. “Crystal Structures of Phosphite Dehydrogenase Provide Insights into Nicotinamide Cofactor Regeneration.” Biochemistry 51.21 (2012): 4263–4270. Print.

Hung, John et al. “Investigation of the Role of Arg301 Identified in the X-Ray Structure of Phosphite Dehydrogenase.” Biochemistry 51.21 (2012): 4254–4262. Print.

Pourmir, Azadeh, and Tyler Johannes. “Directed Evolution: Selection of the Host Organism.” Computational and structural biotechnology journal 2.3 (2012): 1–7. Print.

Johannes, Tyler et al. “Deciphering the Late Biosynthetic Steps of Antimalarial Compound FR-900098.” Chemistry & biology 17.1 (2010): 57–64. Print.

Eliot, Andrew et al. “Cloning, Expression, and Biochemical Characterization of Streptomyces Rubellomurinus Genes Required for Biosynthesis of Antimalarial Compound FR900098.” Chemistry & biology 15.8 (2008): 765–770. Print.

Johannes, Tyler, Ryan Woodyer, and Huimin Zhao. “High-Throughput Screening Methods for Oxidoreductases.” Protein Science Encyclopedia (2008): n. pag. Print.

Choi, Yoo et al. “Heterologous Production of Spectinabilin and Analysis of the Biosynthesis Gene Cluster.” 한국생물공학회 학술대회 (2008): 43–43. Print.

McLachlan, Michael, Tyler Johannes, and Huimin Zhao. “Further Improvement of Phosphite Dehydrogenase Thermostability by Saturation Mutagenesis.” Biotechnology and bioengineering 99.2 (2008): 268–274. Print.

DeSieno, Matthew, Tyler Johannes, and Huimin Zhao. “Deciphering the Late Steps In FR900098 Biosynthesis.” The 2008 Annual Meeting (2008): n. pag. Print.

Nair, Nikhil et al. “Biobutanol from Yeast: a Synergistic Genome and Protein Engineering Approach.” The 2008 AIChE annual meeting, Philadelphia, PA (2008): n. pag. Print.

Eliot, Andrew et al. “Biosynthesis of the Potent Antimalarial Compound FR900098.” Chemistry & biology 15.8 (2008): 765. Print.

Johannes, Tyler, Ryan Woodyer, and Huimin Zhao. “Efficient Regeneration of NADPH Using an Engineered Phosphite Dehydrogenase.” Biotechnology and bioengineering 96.1 (2007): 18–26. Print.

Johannes, Tyler, Ryan Woodyer, and Huimin Zhao. “High-Throughput Screening Methods Developed for Oxidoreductases.” Enzyme Assays (2006): 77. Print.

Woodyer, Ryan, Tyler Johannes, and Huimin Zhao. “Regeneration of Cofactors for Enzyme Biocatalysis.” Enzyme technology (2006): 83–101. Print.

Johannes, Tyler, and Huimin Zhao. “Directed Evolution of Enzymes and Biosynthetic Pathways.” Current opinion in microbiology 9.3 (2006): 261–267. Print.

Johannes, Tyler, Ryan Woodyer, and Huimin Zhao. “Directed Evolution of a Thermostable Phosphite Dehydrogenase for NAD (P) H Regeneration.” Applied and environmental microbiology 71.10 (2005): 5728–5734. Print.

Book Chapter

Johannes, Tyler, Michael Simurdiak, and Huimin Zhao. “Biocatalysis.” Encyclopedia of Chemical Processing. 2006. 101–110. Print.

Technical Report

Crunkleton, Daniel et al. Development of Green Fuels From Algae-The University of Tulsa. The University of Tulsa, Tulsa, OK, 2012. Print.

Conference Proceeding

Hawrylak, Peter et al. “HydroSense: A Self-Powered Wireless Device for Monitoring Water Usage in Hotel Showers.” Global Humanitarian Technology Conference (GHTC), 2015 IEEE. 2015. 314–320. Print.


Zhao, Huimin et al. “Oxidoreductase Comprising Enhanced Thermostability for Use as Nicotinamide Adenine Dinucleotide Phosphate Regenerative Tool.” (2008): n. pag. Print.

Zhao, Huimin, Michael McLachlan, and Tyler Johannes. “Engineered Phosphite Dehydrogenase Mutants.” (2008): n. pag. Print.

Johannes, Tyler. “Directed Evolution of Phosphite Dehydrogenase and Engineered Biosynthesis of FR-900098.” UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN (2008): n. pag. Print.



CHE 4013 Chemical Engineering Lab II
CHE 4063 Chemical Reactor Design
CHE 4173 Biochemical Engineering
CHE 4981 Undergraduate Research
CHE 7003 Fluid Mechanics
CHE 7913 Master's Project
CHE 9981 Research and Dissertation
ES 3003 Introductory Fluid Mechanics
ES 3073 Heat Transfer